Expression of Regulated Oncogen-Alpha by Primary Hepatocytes Following Isolation and Heat Shock Stimulation

نویسندگان

  • Ali Esmailizadeh Koshkoieh Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
  • Azam Torabi Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
  • Azim Mousavizadeh Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
  • Heydar Ghiasi Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
  • Mohammad Reza Nassiry Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
  • Mohammadreza Mohammad Abadi Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
چکیده مقاله:

High levels of regulated oncogen-alpha (GRO-a) expression have been observed in the liver. GRO-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. Given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. In this study, hepatocytes (2×106 cell/ml) were isolated from male Sprague Dawley rat liver and cultured on plates that were pre-coated with collagen type-I matrix. The western and northern blot analyses were employed to detect GRO-a at the protein and mRNA levels in freshly isolated and cultured hepatocytes in response to isolation and heat shock stresses. GRO-a was shown to be expressed by isolated rat hepatocytes immediately after isolation and early culture and decreased with time. mRNA was also expressed in freshly isolated cells (0 h) and did not decrease after 48h of culture and further time points (P<0.01). These results also demonstrated that expression of GRO-a by hepatocytes increased in response to heat shock at different time points in comparison with the control (P<0.01). These results demonstrated that the isolation and heat shock stresses induced the expression of GRO-a in hepatocytes in a time-dependent manner. Thus, it seems that hepatocytes mimic the experiences that the liver encounters after injury in vivo. In such a situation, liver produces stress related agents like chemokines to overcome injurious conditions. 

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

expression of regulated oncogen-alpha by primary hepatocytes following isolation and heat shock stimulation

high levels of regulated oncogen-alpha (gro-a) expression have been observed in the liver. gro-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. in this study, hepatocytes (2×106 cell/ml) were...

متن کامل

expression of stromal derived factor alpha (sdf-1α) by primary hepatocytes following isolation and heat shock stimulation

stromal derived factor-a (sdf-1-a) is a cxc chemokine which has been demonstrated as a recruitment factor for leukocytes to the site of inflammation, infection, injury and following stress. this chemokine has been shown to be expressed by liver cells and in liver diseases. hence, the aim of this study was to examine the expression of sdf-1 by hepatocytes in responses to the stress imposed durin...

متن کامل

Expression of CXC chemokine IP-10/Mob-1 by primary hepatocytes following heat shock.

OBJECTIVE To examine the expression of chemokine IP-10/Mob-1 of hepatocytes in responses to the stress imposed during isolation by collagenase perfusion. METHODS This study was performed in the Faculty of Life Sciences University of Manchester during 2001-2005. We employed western and northern blotting analysis to detect IP-10/Mob-1 in isolated and cultured hepatocytes in response to isolatio...

متن کامل

Activation of hepatocytes by extracellular heat shock protein 72.

Heat shock protein (HSP) 72 is released by cells during stress and injury. HSP-72 also stimulates the release of cytokines in macrophages by binding to Toll-like receptors (TLR) 2 and 4. Circulating levels of HSP-72 increase during hepatic ischemia-reperfusion injury. The role of extracellular HSP-72 (eHSP-72) in the injury response to ischemia-reperfusion is unknown. Therefore, the objective o...

متن کامل

The levels of retinoic acid-inducible gene I are regulated by heat shock protein 90-alpha.

Retinoic acid-inducible gene I (RIG-I) is an intracellular pattern recognition receptor that plays important roles during innate immune responses to viral dsRNAs. The mechanisms and signaling molecules that participate in the downstream events that follow activation of RIG-I are incompletely characterized. In addition, the factors that define intracellular availability of RIG-I and determine it...

متن کامل

Constitutive heat shock protein 70 (HSC70) expression in rainbow trout hepatocytes: effect of heat shock and heavy metal exposure.

The 70-kDa family of heat shock proteins plays an important role as molecular chaperones in unstressed and stressed cells. The constitutive member of the 70 family (hsc70) is crucial for the chaperoning function of unstressed cells, whereas the inducible form (hsp70) is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. In fish, th...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 7  شماره 1

صفحات  1- 9

تاریخ انتشار 2009-01-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023